Esculin-based medium for isolation and identification of Cryptococcus neoformans
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چکیده
منابع مشابه
Esculin-based medium for isolation and identification of Cryptococcus neoformans.
A simple medium was developed, using esculin as the substrate, for the isolation and identification of Cryptococcus neoformans. C. neoformans produced a brown-black pigment on the medium; all other yeasts produced no pigment or were light yellow. Esculin is beta-glucose-6,7-dihydroxycoumarin. C. neoformans produced pigment because the 6,7-dihydroxycoumarin component of the esculin molecule was ...
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The isolation and identification of Cryptococcus neoformans is improved by use of a potato dextrose medium containing urea-antibiotic supplements and a pH of 3.5.
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Cryptococcus neoformans was isolated from nine pathological specimens cultured for mycobacteria. Five of these were recovered only in liquid TBC medium in the absence of growth on conventional substrates.
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Four media, Staib's Guizotia abyssinica, trypan blue, and Staib's with 2 and 10 mg of methyl violet per liter, were compared for the selective and differential isolation of Cryptococcus neoformans from environmental samples. Trypan blue medium allowed for the differentiation of C. neoformans colonies from Candida albicans colonies several days earlier than did Staib's medium. The addition of me...
متن کاملA new caffeic acid minimal synthetic medium for the rapid identification of Cryptococcus neoformans isolates.
Melanin production is one of the most important criteria for rapid identification of Cryptococcus neoformans. Most of the media described in the literature for identifying C. neoformans are very complex; they contain many organic or inorganic compounds and are difficult to prepare and store. The new minimal synthetic caffeic acid medium described in this paper is simpler to prepare, convenient ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1980
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.12.3.332-335.1980